Contact an Account ManagerDirect resolution of the stereoisomers of leucovorin and 5-methyltetrahydrofolate using a bovine serum albumin HPLC chiral stationary phase coupled to an achiral phenyl column
Summary
Type: HPLC
Application #: 105870
Catalog#: 720046.40 - MN HPLC Column, Resolvosil BSA-7, 300A, 7um, 4x150mm
Detection: UV, 310nm, 0.010 AUFS
Conditions: eluent: 0.25M phosphate buffer pH 5.0
flow rate 0.5ml/min
temperature 40°C
Authors: Wainer IW
Source: J Chromatogr 424 (1988) 158 - 162
Details
Legend: Fig. 3. Representative chromatograms on RESOLVOSIL® BSA-7 after switching from
the achiral system: A) leucovorin serum standard (419ng/ml per isomer), B)
5-methyltetrahydrofolate serum standard (419